Urinary Vitamin C Test Kit (1 tablet)

Urinary Vitamin C Test Kit (1 tablet)

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URINARY VITAMIN C TEST KIT

CONTENTS:
1-Dichlorophenolindophenol (DPLP) tablet
1-Glass amber 50ml bottle
3-Glass test tubes
2-Pipettes
Instruction pamphlet on the test
MSDS safety information sheets

WARNINGS AND PRECAUTIONS:
Skin or eye contact may result in irritation and may be harmful if inhaled or ingested. Refer to Dichlorophenolindophenol MSDS sheet in cases of eye or skin contact, ingestion or inhalation and seek medical attention.

STORAGE:
Store solution in light resistant bottle at 2 - 8ยบ C. Under these conditions the solution is stable for up to 6 months. Discard if the solution fades from its original Royal Blue color.

RECOMMENDED USE:
Determining the approximate concentration of Vitamin C in urine samples.

RECOMMENDED HANDLING PROCEDURES:
Wear gloves and eye protection while handling the tablets or solution and avoid spills.

TEST PROCEDURE:

1. Prepare DL Test Solution

a. Using a 50ml dropper bottle add one Dichlorophenolindophenol tablet and 30ml of distilled water.
b. Allow the tablet to completely dissolve by letting the solution sit overnight.
c. Once the tablet is completely dissolved, ensure the solution is Royal Blue in color. If not discard
and prepare a new solution using a new tablet and clean 50ml dropper bottle.

2. Vitamin C Test

a. Into a clean test tube add 5 drops of the blue DL Test Solution. If the blue color fades in the test
tube then discard and start with a new, clean test tube.
b. To the test tube add urine, one drop at a time, and count the drops required to turn the blue
solution to clear.
c. Refer to the table (included with test) for results interpretation.


1) Reference: The Reliability of the Method for Estimating Vitamin C by Titration against 2:6
-DPLP, Harris and Olliver, University of Cambridge, 1941.
2) Assuming standard conditions and an endpoint equal to 0.52875mg of Ascorbic Acid for 5 drops of the prepared DPLP solution.

LIMITATIONS:
Other oxidizing agents (besides Vitamin C) in the urine may also reduce the DPLP and cause a falsely high result.

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